Immunoperoxidase staining of leptospires.
نویسندگان
چکیده
Leptospires were detectable by light microscopy when Formalin-fixed preparations were reacted by direct and indirect methods with horseradish peroxidase-labeled specific antibody.
منابع مشابه
Rapid detection of herpes simplex virus in clinical specimens by centrifugation and immunoperoxidase staining.
The effect of immunoperoxidase staining and centrifugation on the sensitivity and rapidity of herpes simplex virus detection in mink lung cell cultures was determined with 730 clinical specimens. In standard tube cultures, the use of immunoperoxidase staining resulted in detection of 31 (91%) of 34 positive cultures after overnight incubation, compared with 25 (74%) detected without the stain (...
متن کاملImmunoperoxidase staining of formalin-fixed, paraffin-embedded, human renal biopsies with a comparison of the peroxidase-antiperoxidase (PAP) and indirect methods.
The results of immunoperoxidase staining of 33 routinely processed renal biopsies correlated with the results of direct immunofluorescence in 79% of tests performed. Most of the discrepant results were due to positive immunoperoxidase staining, possibly reflecting greater sensitivity of the method. A comparison of two immunoperoxidase methods showed that the indirect method produced less intens...
متن کاملModified toluidine blue: an alternative stain for Helicobacter pylori detection in routine diagnostic use and post-eradication confirmation for gastric cancer prevention.
BACKGROUND Modified toluidine blue staining (MTBs) is a simple, inexpensive and time saving method to detect H. pylori in gastric biopsy specimens. As a metachromatic stain, it simultaneously highlights intestinal metaplasia, a gastric cancer precancerous lesion. The aim of this study was to assess the reliability of MTBs compared with hematoxylin-eosin (H and E) for H. pylori detection using i...
متن کاملDetection of Leptospira in soil and water by immunofluorescence staining.
Specific immunofluorescence staining was used for the detection of leptospires in soil and water under laboratory and field conditions.
متن کاملRapid immunoperoxidase staining of axons for frozen section diagnosis of nerve biopsy specimens.
A new method of freezing and embedding a nerve biopsy specimen and staining it with the immunoperoxidase technique for neurofilaments was developed to overcome the difficulties normally encountered in the assessment of tiny portions of nerve. The method clearly shows the architecture of the nerve, the exact number and size of all axons present, and the degree of fibrosis present. The entire pro...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Applied microbiology
دوره 27 1 شماره
صفحات -
تاریخ انتشار 1974